One Factor at A Time based optimization of protease from poultry associated Bacillus licheniformis
نویسندگان
چکیده
منابع مشابه
Statistical Approach for Optimization of Physiochemical Requirements on Alkaline Protease Production from Bacillus licheniformis NCIM 2042
The optimization of physiochemical parameters for alkaline protease production using Bacillus licheniformis NCIM 2042 were carried out by Plackett-Burman design and response surface methodology (RSM). The model was validated experimentally and the maximum protease production was found 315.28 U using optimum culture conditions. The protease was purified using ammonium sulphate (60%) precipitatio...
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Alkaline Protease enzyme produced from Bacillus Licheniformis PTCC1331 in optimum Condition(Temperature 37 ?C, 150 rpm, PH=10), is immobilized in different concentrations of Sodium Alginate using entrapment method, to increase its thermal resistance in detergents, and its activity is measured thereafter. Maximum enzymatic activity of 46 was obtained in 2%w/v Sodium alginate solution. Investiga...
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Keratin are insoluble fibrous proteins found in hair, wool, feather, nail, horns and other epithelial covering which is rich in beta helical coil linked through cysteine bridges. Keratinase (EC 3.4.4.25) belongs to the class hydrolase which are able to hydrolyse insoluble keratins more efficient than other proteases. The bacteria Bacillus licheniformis showing higher keratinase activity was scr...
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The production of protease by Bacillus licheniformis BBRC 100053 was studied. The most appropriate medium for the growth and protease production is composed of: lactose 1%, yeast extract 0.5%, peptone 0.5%, KH2PO4 0.1%, MgSO4.7H2O 0.02%. Enzyme production corresponded with growth and reached a maximums level (589 U/ml) during the stationary phase at 35°C, pH equivalent to 10 and with 150 rpm af...
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The aim of this study was to optimize protease production using a novel Bacillus sp. ZR-5 strainisolated from the soil, and evaluate its application in detergent industry. The proteolytic activityof the strain was demonstrated using gelatin hydrolysis screening test. Protease productionoptimization was carried out using a two-step approach: a conventional method in order toidentify the best car...
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ژورنال
عنوان ژورنال: Journal of Applied Pharmaceutical Science
سال: 2016
ISSN: 2231-3354
DOI: 10.7324/japs.2016.60315